![]() Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. For detection of any western blot, it is desirable to use prestained molecular weight markers (also called protein ladders) that are transferred to the membrane along with the protein sample. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. Towbin et al in 1979 ( Towbin, Staehelin, & Gordon. It is built on a technique that involves transferring, also known as blotting, proteins separated by electrophoresis from the gel to a membrane where they can be visualized specifically. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. Western Blot (WB) is a common method to detect and analyze proteins. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr). Publishing research using ab116027? Please let us know so that we can cite the reference in this datasheet.Īb116027 has been referenced in 5 publications. Review other protein ladders in the unstained and prestained protein ladder guide. Recommended loading: ~2 - 3 µl for western blots. ![]() Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.Ready-to-use: Supplied in a loading buffer for direct loading on gels.The ladder is supplied in gel loading buffer and is ready to use.ĭo not heat, dilute, add reducing agent before loading. This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.
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